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Description

This study of the zebrafish eye is a series of four images taken at increasing magnifications. This image, the first image is the SEM block face of the zebrafish eye. This is followed by a second image that is a higher magnification TEM image of the plexiform layer and the outer nuclear layer of the zebrafish retina. The third image is a 15,000X TEM image of the bipolar and horizontal cells interdigitating in the OPL layer of the zebrafish retina. The fourth image in the series is a higher magnification (60,000X) of the dense synaptic bar and synaptic vesicles. This image of the larval zebrafish eye 10dpf was collected on a Gatan 3View® serial block face scanning electron microscope (SBFSEM). The 3View® is attached to a FEI Quanta 600 FEG SEM. Block face imaging is a way to look at epon embedded specimens using backscattered electrons in the SEM. A diamond knife in the vacuum chamber of the SEM cuts hundreds of ultrathin serial sections, thus gathering a perfected registered stack of images. Reference for SBFSEM: Denk, W. and H. Horstmann. Serial block face scanning electron microscopy to reconstruct three- dimensional tissue nanostructure. PloS Biology vol 2, 11; e329 doi:10.1371/journal.pbio.0020329 Microwave processing, osmium tetroxoxide/osmid procedure for enhancing membranes, and copper lead en bloc staining. Procedure for specimen preparation available at http://www.stanford.edu/~redhair/JoAnn_Buchanan.

Biological Sources

NCBI Organism Classification
Danio rerio
(zebrafish)
Cell Type
FMA:54448

Biological Context

Biological Process
eye morphogenesis
Zebrafish Anatomy
retina
eye

Attribution

Names
JoAnn Buchanan
Christel Genoud
Link
http://www.stanford.edu/~redhair/JoAnn...

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
serial block face SEM (SBFSEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
lead salt
copper salt
Processing History
unprocessed raw data

Dimensions

Spatial Axis Image Size Pixel Size
X 750px .333μm
Y 750px .333μm
*CIL – Cell Image Library accession number. Please use this to reference an image.