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Licensing
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:48105*  Cite 
Description

Cryopreserved rat astrocytes were revived and stained for GFAP (in red) to reveal cytoskeleton and DAPI (in blue) to label nuclei. GFAP mutations and intracellular accumulation have been proposed to play an important role in a rare genetic disorder, Alexander Disease.

Technical Details

Rat astrocytes were fixed and immunostained for GFAP (from Stem Cell Technologies), and DAPI (from Life Technologies). Images were acquired with a 40X (Plan Fluor, NA 0.75) lens, SPOT RT Slider CCD camera and SPOT Basic image capture software. Image generated with the Image J color combine function.

Biological Sources
NCBI Organism Classification
Rattus norvegicus
Cell Type
astrocyte
Cellular Component
intermediate filament
nucleus
Attribution
Names
Natalie Prigozhina
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL48105
Archival Resource Key (ARK)
ark:/b7295/w9cil48105
Sample Preparation
Methods
Dispersed cells cryopreserved
revived and cultured in vitro
Dimensions