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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:47953*  Cite 
Description

The electron micrograph shows the electron dense basal body in a testis cell in primary culture.

Technical Details

Testes from 15-day-old Sprague-Dawley rats (Sasco, Omaha Neb.) were dissociated in 0.1% trypsin and plated in NCTC-135 in Petri dishes containing cover slips. Cells on cover slips were fixed in 2.5% glutaraldehyde, 1% osmium tetroxide, stained en bloc with uranyl acetate, dehydrated and embedded in Araldite. Sections were taken parallel to the cover slip, stained with uranyl acetate and lead citrate and examined with a Philips 300 transmission electron microscope. Images were captured on film, printed onto photographic paper, digitized with a scanner and processed with Photoshop (Adobe).

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
testis cell
Attribution
Names
Dennis Diener
Link
Trends in Cell Biology
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL47953
Archival Resource Key (ARK)
ark:/b7295/w9cil47953
Grouping This image is part of a group.
Imaging
Image Mode
recorded image
Parameters Imaged
electron micrograph
Dimensions
Spatial Axis Image Size Pixel Size
X 4546px ——
Y 5671px ——