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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:47049*  Cite 

The purpose of this study was to investigate the progression of changes in retinal ganglion cells and optic nerve glia in neurofibromatosis-1 (NF1) genetically-engineered mice with optic glioma. RGC were counted in whole retina preparations using high-resolution, mosaic confocal microscopy following their delineation by retrograde FluoroGold labeling. Then, we found reduced RGC numbers in Nf1+/GFAPCKO mice, supporting a model in which the combination of optic nerve Nf1 heterozygosity and glial cell Nf1 loss results in disrupted axonal-glial relationships, subsequently culminating in the degeneration of optic nerve axons and loss of their parent RGC neurons.

Technical Details

Specimen was imaged using a Olympus DSU Confocal Microscope with the Olympus 20x 1.4 NA objective.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
retinal ganglion cell
Cellular Component
cell body
Keun-Young Kim
David Gutmann
CCDB 7732
Eric Bushong
Mark Ellisman
Da-Yong Lee
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 14000px 0.39nm
Y 14000px 0.39nm