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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:46201*  Cite 
Description

Plate 20586 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules. Images The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay). This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

Technical Details

Gustafsdottir et al. (doi:10.1371/journal.pone.0080999) have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery. Using the cell-painting assay, the Broad Institute has assembled a reference dataset of profiles for U2OS osteosarcoma cells treated with ~30,000 compounds. The compound collection includes DOS-derived compounds (20,000), as well as chemically diverse MLI compounds with biologically diverse performance identified through analysis of PubChem (10,000), and known bioactive compounds to serve as landmarks (2,500). The DOS compounds consist of structurally diverse and stereochemically rich compounds with structures distinct from the current MLSMR. The compound collection also includes 267 distinct compounds nominated by MLPCN Centers from projects for which the Centers would like to identify new chemical series with similar activities. The experiment consists of 413 microtiter plates. Each plate has 384 wells. Each well has 9 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale 16-bit TIFF image file.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Line
U-2 OS
Cellular Component
cell
Biological Context
Biological Process
response to chemical stimulus
Attribution
Names
Sigrun Gustafsdottir
Vebjorn Ljosa
Katherine Sokolnicki
Joshua Bittker
Nicole Bodycombe
Mark Bray
Vlado Dancik
Tom Hasaka
Suk Hon
Melissa Kemp
Kejie Li
Deepika Walpita
Mathias Wawer
Todd Golub
Stuart Schreiber
Paul Clemons
Anne Carpenter
Alykhan Shamji
Link
Broad Institute
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL46201
Archival Resource Key (ARK)
ark:/b7295/w9cil46201
Compounds
1-[1-(2-BENZO[b]THIENYL)CYCLOHEXYL)]PIPERIDINE
1-(1-NAPHTHYL)PIPERAZINE
1-(3-CHLOROPHENYL)PIPERAZINE
1-(3-trifluoromethyl) phenylpiperazine monohydrochloride
(1S,9R)-beta-HYDRASTINE
2,5-ditertbutylhydroquinone
(+ -)-2-(N-PHENYLETHYL-N-PROPYL)AMINO-5-HYDROXYTETRALIN)
3'-FLUOROBENZYLSPIPERONE
3-HYDROXYMETHYL-beta-CARBOLINE
5-alpha-PREGNAN-3-alpha-OL-20-ONE
5-CHLOROINDOLE-2-CARBOXYLIC ACID
5-iodotubercidin
7-CHLOROKYNURENIC ACID
7-HYDROXY-DPAT
A 68930
A-77636
Acacetin
Acemetacin
AG-370
AJ-76
Ala-Ala-Phe-CMK
Alclometasone dipropionate
Alfuzosin hydrochloride
alpha-ERGOCRYPTINE
Alprenolol hydrochloride
AM 92016
Aminopurine, 6-benzyl
Anabasine
Austricine hydrate
B581
(+ -)-BACLOFEN
BAPTA-AM
Bendroflumethiazide
Benzocaine
Benzydamine hydrochloride
Bestatin
Betulinic acid
B-HT 920
BP 554
BRL 15572
Bupivacaine hydrochloride
Bupropion hydrochloride
(+)-BUTACLAMOL
Butamben
BUTYL-beta-CARBOLINE-3-CARBOXYLATE
BW-B 70C
Cabergoline
Carbinoxamine maleate salt
CDC
Cefoperazone dihydrate
Cefuroxime sodium salt
Cephalothin sodium salt
Cerulenin
CGP 52432
CHLORMETHIAZOLE
CHLORMEZANONE
Chlorphensin carbamate
Chlorpropamide
Cinnarizine
Cinoxacin
cis-(Z)-FLUPENTHIXOL
CL 218872
Clemastine fumarate
Clobetasol propionate
Clofibric acid
CLOMIPRAMINE
Clonidine hydrochloride
Cloperastine hydrochloride
CLOZAPINE
Curcumin
Cycloheximide
cyclopamine
cytochalasin D
DAIDZEIN
Dantrolene sodium salt
Deferoxamine mesylate
Deltaline
Demeclocycline hydrochloride
Deprenyl
Dicumarol
Diethylcarbamazine citrate
DIHYDROERGOCRISTINE
DIINDOLYLMETHANE
Dilazep dihydrochloride
Diperodon hydrochloride
Diphenidol hydrochloride
Diphenylpyraline hydrochloride
DIPROPYLDOPAMINE
Dipyrone
Disopyramide
(d,l)-Tetrahydroberberine
Doxepin hydrochloride
Drofenine hydrochloride
Dropropizine (R,S)
Dydrogesterone
Econazole nitrate
ERGOCORNINE
ESTRADIOL
Estropipate
Ethacrynic acid
Ethisterone
Ethoxyquin
ETHYL-beta-CARBOLINE-3-CARBOXYLATE
Etodolac
Eucatropine hydrochloride
Evoxine
Famprofazone
Fenbendazole
Fenbufen
Fendiline hydrochloride
Fenofibrate
FENOLDOPAM
Fenoterol hydrobromide
Fenspiride hydrochloride
FG 7142
FGIN-1-27
Fillalbin
Finasteride
Fipexide hydrochloride
Flavoxate hydrochloride
Flecainide acetate
FLUMAZENIL
Flumequine
Flumethasone
Flunarizine dihydrochloride
Flunixin meglumine
Fluocinonide
Fluorocurarine chloride
Fluorometholone
FLUSPIRILINE
Foliosidine
Furaltadone hydrochloride
Gabazine
GBR-12909
GBR 12935
Gelsemine
Gemfibrozil
GF-109203X
Glafenine hydrochloride
GR 55562
Graveoline
Guaifenesin
HALOPERIDOL
Heliotrine
Hexestrol
Hexylcaine hydrochloride
Hoechst 33342 (cell permeable) (BisBenzimide)
Homochlorcyclizine dihydrochloride
Hyoscyamine (L)
Ifenprodil tartrate
Indapamide
Isoetharine mesylate salt
Karakoline
Kawain
Ketoprofen
KN-62
L-741,742
L-745,870
L-750,667
Latrunculin B
Lavendustin A
Leflunomide
LFM-A13
Lincomycin hydrochloride
Lisinopril
Lomefloxacin hydrochloride
LY-163,502
LY-165,163
Lycorine hydrochloride
MCI-186
Mebendazole
Mebeverine hydrochloride
Meclofenamic acid sodium salt monohydrate
Meclofenoxate hydrochloride
Medrysone
Mefexamide hydrochloride
MESORIDAZINE
METERGOLINE PHENYLMETHYL ESTER
Methazolamide
METHYL-6,7-DIMETHOXY-4-ETHYL-beta-CARBOLINE-3-CARBOXYLATE
METHYL-beta-CARBOLINE-3-CARBOXYLATE
METHYLERGOMETRINE
Methylprednisolone, 6-alpha
Metoclopramide monohydrochloride
Mevinolin (lovastatin)
Mexiletine hydrochloride
Midodrine hydrochloride
N-(4-BROMOBENZYL)-5-METHOXYTRYPTAMINE
NAN-190
N-DESMETHYLCLOZAPINE
Nefopam hydrochloride
Nifenazone
NIMODIPINE
Nitrarine dihydrochloride
Norethindrone
Norfloxacin
Norgestrel-(-)-D
Nortriptyline hydrochloride
N-PHENYLANTHRANILIC
NPPB
NS-1619
NSC-95397
Ofloxacin
Oxybutynin chloride
PAXILLINE
PCA 4248
PENITREM A
Pentoxifylline
PERGOLIDE METHANESULFONATE
Phenethicillin potassium salt
Phentolamine hydrochloride
PHENYTOIN
Piceatannol
PICROTOXININ
pifithrin
Pimethixene maleate
PIMOZIDE
PINACIDIL
Piperacillin sodium salt
PIRIBEDIL
Piromidic acid
PK-11195
PNU 22394 HCl
PNU 96415E
Podophyllotoxin
PP1
Pramoxine hydrochloride
Praziquantel
Prednisolone
Pridinol methanesulfonate salt
Prilocaine hydrochloride
PRIMIDONE
Proglumide
PROPAFENONE
PROPOFOL
PROPYL-beta-CARBOLINE-3-CARBOXYLATE
Pyrilamine maleate
Pyrithyldione
Quinacrine dihydrochloride dihydrate
(-)-QUINPIROLE
QUIPAZINE
QX-314
R(+)-6-BROMO-APB
R(-)-APOMORPHINE
REMOXIPRIDE
resveratrol
Ricinine
RILUZOLE
RISPERIDONE
R(-)-N-ALLYLNORAPOMORPHINE
Ropinirole HCl
R(-)-PROPYLNORAPOMORPHINE
R(+)-SKF-81297
(RS)-(+ -)-SULPIRIDE
SACLOFEN
Salbutamol
SB 202190
SB 216641
SB-415286
SB-431542
Scopolamin-N-oxide hydrobromide
SDZ-201106
SDZ SER 082
SKF 89976A
SKF-96365
SPIROXATRINE
Splitomycin
S(-)-SULPIRIDE
staurosporine
STAUROSPORINE
Strophanthidin
Succinylsulfathiazole
Sulfabenzamide
Sulfachloropyridazine
Sulfadimethoxine
Sulfamerazine
Sulfameter
Sulfamethazine sodium salt
Sulfamethizole
Sulfamethoxypyridazine
Sulfapyridine
Sulfinpyrazone
Tanshinone IIA
Telenzepine dihydrochloride
Terazosin hydrochloride
Tetrahydrozoline hydrochloride
Tinidazole
TMB-8
TOLAZAMIDE
TOLBUTAMIDE
Tolfenamic acid
TRANS-7-HYDROXY-PIPAT
TRIFLUOPERAZINE
Trihexyphenidyl-D,L Hydrochloride
Trimethoprim
Trioxsalen
Triprolidine hydrochloride
Triptolide
Tropicamide
tyrphostin 1
TYRPHOSTIN 46
U-0126
U-50488
U-74389G
U-99194A
Ungerine nitrate
VERAPAMIL
VERATRIDINE
vinblastine
Vinpocetine
Wiskostatin
YS035
ZM226600
ZM336372
Zomepirac sodium salt