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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:45758*  Cite 
Description

Plate 20595 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules. Images The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay). This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

Technical Details

Gustafsdottir et al. (doi:10.1371/journal.pone.0080999) have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery. Using the cell-painting assay, the Broad Institute has assembled a reference dataset of profiles for U2OS osteosarcoma cells treated with ~30,000 compounds. The compound collection includes DOS-derived compounds (20,000), as well as chemically diverse MLI compounds with biologically diverse performance identified through analysis of PubChem (10,000), and known bioactive compounds to serve as landmarks (2,500). The DOS compounds consist of structurally diverse and stereochemically rich compounds with structures distinct from the current MLSMR. The compound collection also includes 267 distinct compounds nominated by MLPCN Centers from projects for which the Centers would like to identify new chemical series with similar activities. The experiment consists of 413 microtiter plates. Each plate has 384 wells. Each well has 9 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale 16-bit TIFF image file.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Line
U-2 OS
Cellular Component
cell
Biological Context
Biological Process
response to chemical stimulus
Attribution
Names
Sigrun Gustafsdottir
Vebjorn Ljosa
Katherine Sokolnicki
Joshua Bittker
Nicole Bodycombe
Mark Bray
Vlado Dancik
Tom Hasaka
Suk Hon
Melissa Kemp
Kejie Li
Deepika Walpita
Mathias Wawer
Todd Golub
Stuart Schreiber
Paul Clemons
Anne Carpenter
Alykhan Shamji
Link
Broad Institute
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL45758
Archival Resource Key (ARK)
ark:/b7295/w9cil45758
Compounds
11alpha-ACETOXYKHIVORIN
1- (2-METHOXYPHENYL)PIERAZINE HYDROCHLORIDE
1,3-DIDEACETYL-7-DEACETOXY-7-OXOKHIVORIN
1,3-DIDEACETYLKHIVORIN
1R,2S-PHENYLPROPYLAMINE
1R,9S-HYDRASTINE
2',4'-DIHYDROXYCHALCONE 4'-GLUCOSIDE
2,4-DINITROPHENOL
2',5'-DIHYDROXY-4-METHOXYCHALCONE
2,6-DIHYDROXY-4-METHOXYTOLUENE
2',beta-DIHYDROXYCHALCONE
2-HYDROXYXANTHONE
2-METHYL-3-HYDROXYETHYLENEPYRAN-4-ONE
3-DEOXO-3beta-ACETOXYDEOXYDIHYDROGEDUNIN
3-DEOXY-3beta-HYDROXYANGOLENSIC ACID METHYL ESTER
3-METHYLCHOLANTHRENE
4-HYDROXY-6-METHYLPYRAN-2-ONE
4'-HYDROXYCHALCONE
4'-METHOXYFLAVONE
6,3'-DIMETHOXYFLAVONE
6-HYDROXYANGOLENSIC ACID METHYL ESTER
7,8-DIHYDROXYFLAVONE
7-CHLOROETHYLTHEOPHYLLINE
7-DEACETOXY-7-OXODEOXYGEDUNIN
7-METHOXYCHROMONE
7-NITROINDAZOLE
8-HYDROXY-15,16-BISNOR-11-LABDEN-13-ONE
8-HYDROXYCARAPINIC ACID
ACETYLSEROTONIN
ACETYL TYROSINE ETHYL ESTER
ACTINONIN
AESCULIN
ALACHLOR
ANDIROBIN
ANGOLENSIC ACID, METHYL ESTER
ATOVAQUONE
AVERMECTIN B1
Azacyclonol
AZINPHOS METHYL
Azlocillin sodium salt
Bacampicillin hydrochloride
BENAZEPRIL HYDROCHLORIDE
BENZANTHRONE
BENZYL PENICILLIN POTASSIUM
beta-DIHYDROROTENONE
BETAMETHASONE VALERATE
BIFONAZOLE
Boldine
BOLDINE
BROMOCRIPTINE MESYLATE
Bromopride
BRUCINE
BUDDLEOFLAVONOLOSIDE
CAFFEINE
CANDESARTAN CILEXTIL
CATECHIN PENTABENZOATE
Cefaclor
Cefamandole sodium salt
CEFOTAXIME SODIUM
CELECOXIB
CHLORAMPHENICOL
CHLORPHENIRAMINE (S) MALEATE
CHLORPYRIFOS
CHRYSANTHEMIC ACID
CHRYSIN DIMETHYL ETHER
CHRYSOPHANOL
Cinchonine
CINCHONINE
Clomiphene citrate (Z,E)
Clomipramine hydrochloride
CLOPIDOGREL SULFATE
CLOXYQUIN
COMPACTIN
Cortisone
Corynanthine hydrochloride
CRASSIN ACETATE
CYCLOVERATRYLENE
CYPROTERONE ACETATE
DANAZOL
DEACETOXY-7-OXOGEDUNIN
DEACETYLGEDUNIN
DEGUELIN(-)
DEHYDRO (11,12)URSOLIC ACID LACTONE
DEHYDRODIHYDROROTENONE
DEOXYANDIROBIN LACTONE
DEOXYKHIVORIN
DEOXYSAPPANONE B 7,3'-DIMETHYL ETHER ACETATE
DEOXYSAPPANONE B 7,4'-DIMETHYL ETHER
DERACOXIB
DERRUSNIN
DEXAMETHASONE ACETATE
DEXTROMETHORPHAN HYDROBROMIDE
DIBENZOYLMETHANE
Dienestrol
DIGITOXIN
DIGOXIN
DIHYDROSAMIDIN
Dimethisoquin hydrochloride
DIOSMETIN
Dioxybenzone
DISOPYRAMIDE PHOSPHATE
DO 897/99
DOXEPIN HYDROCHLORIDE
DUARTIN (-)
Dyclonine hydrochloride
EMODIN
ENOXOLONE
EPHEDRINE (1R,2S) HYDROCHLORIDE
EPIAFZELECHIN (2R,3R)(-)
Epicatechin-(-)
Epivincamine
EPOXYGEDUNIN
ESTRADIOL
ESTRADIOL ACETATE
ESTRADIOL CYPIONATE
ESTRADIOL DIACETATE
ESTRADIOL VALERATE
Estriol
ESTRIOL
ESTRIOL METHYL ETHER
Estrone
ESTROPIPATE
ETHINYL ESTRADIOL
ETHION
ETHYL 1-BENZYL-3-HYDROXY- 2-OXO[5H]PYRROLE-4-CARBOXYLATE
EUCATROPINE HYDROCHLORIDE
EUPATORIOCHROMENE
EVOXINE
EZETIMIBE
FAMCICLOVIR
FENTHION
FEXOFENADINE
FLAVANONE
FLUDROCORTISONE ACETATE
FLUOCINONIDE
FLUOROMETHOLONE
Fluphenazine dihydrochloride
FRAXETIN
FURAZOLIDONE
FUSIDIC ACID
Gabexate mesilate
GATIFLOXACIN
GEDUNIN
GENTIAN VIOLET
Gossypol
GOSSYPOL-ACETIC ACID COMPLEX
Gramine
HARMALINE
Hesperidin
HEXYLRESORCINOL
Hippeastrine hydrobromide
HOMATROPINE BROMIDE
HOMATROPINE METHYLBROMIDE
Hycanthone
HYDROCORTISONE
HYDROCORTISONE ACETATE
HYDROCORTISONE HEMISUCCINATE
HYDROCOTARNINE
HYDROXYAMPHETAMINE HYDROBROMIDE
HYDROXYPROGESTERONE CAPROATE
HYDROXYZINE PAMOATE
HYMECROMONE METHYL ETHER
HYOSCYAMINE
IBUPROFEN
Imipramine hydrochloride
INDOLE-3-CARBINOL
IPRATROPIUM BROMIDE
IRBESARTAN
IRIDIN
IRIGENIN, DIBENZYL ETHER
ISOBERGAPTENE
Isocarboxazid
Isocorydine (+)
ISOEUGENITOL
ISOPILOCARPINE NITRATE
ISOPIMPINELLIN
ISOTECTORIGENIN TRIMETHYL ETHER
Isoxsuprine hydrochloride
Ketoconazole
KUHLMANNIN
LECANORIC ACID
LEVAMISOLE HYDROCHLORIDE
LEVULINIC ACID, 3-BENZYLIDENYL-
LIMONIN
LINCOMYCIN HYDROCHLORIDE
L-PHENYLALANINOL
LUFENURON
LUPANINE PERCHLORATE
LYCORINE
Lysergol
LYSYLPHENYLALANYLTYROSINE
MARMESIN
MARMESIN ACETATE
MECLOFENAMATE SODIUM
MEDROXYPROGESTERONE ACETATE
MEDRYSONE
MEGESTROL ACETATE
MELOXICAM
MELPHALAN
METACETAMOL
METAXALONE
Methantheline bromide
METHSCOPOLAMINE BROMIDE
Methylergometrine maleate
METHYLORSELLINIC ACID, ETHYL ESTER
METHYLPREDNISOLONE
METOPROLOL TARTRATE
MEXICANOLIDE
MICONAZOLE NITRATE
Mifepristone
MUNDOSERONE
MUNDULONE
N- (3-TRIFLUOROMETHYLPHENYL)PIPERAZINE HYDROCHLORIDE (TFMPP)
NALBUPHINE HYDROCHLORIDE
NAPROXEN(+)
NAPROXOL
NEOPINE
NERIIFOLIN
N-FORMYLMETHIONYLPHENYLALANINE
Nialamide
NITROMIDE
N-METHYLANTHRANILIC ACID
NORETHINDRONE
NOSCAPINE HYDROCHLORIDE
Novobiocin sodium salt
OSTHOL
Oxalamine citrate salt
OXFENDAZOLE
OXICONAZOLE NITRATE
OXYQUINOLINE HEMISULFATE
PEFLOXACINE MESYLATE
PENICILLIN V POTASSIUM
Pergolide mesylate
Perphenazine
PERUVOSIDE
PEUCEDANIN
PHENACYLAMINE HYDROCHLORIDE
Phenelzine sulfate
PHENYLACETOHYDROXAMIC ACID
PHLORETIN
PHLORIDZIN
PHOSALONE
PICROPODOPHYLLOTOXIN
PICROPODOPHYLLOTOXIN ACETATE
PIMPINELLIN
PINOCEMBRIN
PINOSYLVIN
PINOSYLVIN METHYL ETHER
PIOGLITAZONE HYDROCHLORIDE
Pivmecillinam hydrochloride
PRAVASTATIN SODIUM
Proadifen hydrochloride
Prochlorperazine dimaleate
PROCHLORPERAZINE EDISYLATE
PROGESTERONE
PROPACHLOR
PROPIOMAZINE MALEATE
PTERYXIN
QUERCITRIN
QUININE SULFATE
Quipazine dimaleate salt
Ranitidine hydrochloride
RANOLAZINE
REBAMIPIDE
Rescinnamin
RETUSIN 7-METHYL ETHER
RIBOFLAVIN
Rifampicin
RITANSERIN
ROSIGLITAZONE
ROSOLIC ACID
SAPPANONE A DIMETHYL ETHER
Scoulerine
SECNIDAZOLE
SECURININE
SELINIDIN
SERICETIN DIMETHYL ETHER
SILDENAFIL
SINAPIC ACID METHYL ETHER
Skimmianine
SODIUM DEHYDROCHOLATE
SPHONDIN
STICTIC ACID
SULFAMETHAZINE
Sulfaquinoxaline sodium salt
SUMATRIPTAN
Syrosingopine
TEGASEROD
TENIPOSIDE
TERBUTALINE HEMISULFATE
TESTOSTERONE PROPIONATE
TETRAHYDROCORTISONE-3,21-DIACETATE
TETRAHYDROPALMATINE
THIMEROSAL
Thioproperazine dimesylate
THIOTHIXENE
TIOXOLONE
TOTAROL ACETATE
TRIADIMEFON
Trifluoperazine dihydrochloride
Trimipramine maleate salt
TRIPTOPHENOLIDE
Trolox
UMBELLIFERONE
URSINOIC ACID
VALDECOXIB
VALYLTRYPTOPHAN
VENLAFAXINE
VERATRIC ACID
Vincamine
WARFARIN
ZEARALENONE