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Description

Imaging an HIV infected dendritic cell reveals numerous viral particles at the tips of filopodia. Actin-based membrane extensions from the dendritic cell are phallodin stained (red). HIV particles (white) decorate the filopodial terminal ends. To label viral particles, a construct containing eGFP at the C-terminus of HIV matrix was expressed. The nucleus of the cell is stained with DAPI and is represented in cyan.

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Type
dendritic cell
Cellular Component
filopodium

Biological Context

Biological Process
viral infectious cycle
Human Disease
Human immunodeficiency virus infectious disease

Attribution

Name
Stuart Turville
Other
The Kirby Institute, University of New South Wales

Imaging

Image Type
recorded image
Imaging Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
phalloidin
4',6-diamidino-2-phenylindole (DAPI)
TagGFP

Sample Preparation

Methods
chemically fixed tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 873px ——
Y 566px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.