Licensing

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Description

Tomograms of specifically labeled connexin 43 (Cx43) gap junctions expressed in HEK293T. Cx43 was genetically tagged with GPF and APEX, an engineered plant peroxidase that reacts with hydrogen peroxide and diaminobenzidine to create a protein specific stain. Sections are cut perpendicular to the membrane plane (cross-section view).

Biological Sources

Cell Line
HEK-293 482R

Biological Context

Biological Process
cellular component organization
Molecular Function
intercellular communication

Attribution

Names
Gina Sosinsky
Alice Ting
Jeff Martell
Mark Ellisman
Thomas Deerinck
Published
Nature Biotechnology
Link
CCDB 83884

Imaging

Image Type
recorded image
Imaging Mode
FBbi:00000623
Parameters Imaged
electron density

Sample Preparation

Methods
glutaraldehyde fixed tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 3076px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.