Licensing

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Description

Digital holographic microscopy video showing cell division of unlabeled JIMT-1 breast cancer cells. During the video, spanning over 72 hours, one cell divides into three daughter cells (seen on top of the cell cluster in the upper right portion of the image). One of the daughter cell dies, and two cells merge. The height of the cells is color coded by the vertical look up bar on the left of the image.

Technical Details

The JIMT-1 cells were seeded on a Ibidi[TM] (Ibidi Gmbh Martinsried,Germany), one well slide and left to incubate for one day (5% CO2, 90% humidity, 37 degrees C). The Ibidi slide was placed on a Ibidi heating plate, set to 37 degrees C, and onto the stage of the HoloMonitor M3 (Phase Holographic Imaging AB, Lund Sweden). One image every 4th minute was captured for a total of 72h. The Hstudio (Phase Holographic Imaging) software was used to color the images and to create an AVI movie of 8 frames/minute. No further processing was performed on the images.

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Type
carcinoma
Cell Line
JIMT-1
Cellular Component
cell surface

Biological Context

Biological Process
asymmetric cell division
Human Disease
breast cancer

Attribution

Name
Birgit Janicke
Link
Phase Holographic Imaging
Other
Lund, Sweden

Imaging

Image Type
recorded image
Imaging Mode
digital holographic microscopy
Parameters Imaged
Phase shift of the laser light passing through the cell
Processing History
Hstudio software (Phase Holographic Imaging AB)

Sample Preparation

Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 1052px ——
Y 684px ——
Time 4 sec
*CIL – Cell Image Library accession number. Please use this to reference an image.