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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:43351*  Cite 
Description

Shown is a a confocal microscope image of a human gingival fibroblast in culture. Interphase microtubules (green) are labeled with alpha/beta tubulin primary antibodies. FITC conjugated secondary antibody was applied afterwards. The centrosomal protein pericentrin was labeled with anti-pericentrin followed by Cy3 conjugated secondary antibody and appears as a pair of red juxtanuclear signals (red). Nuclear DNA (blue) was stained with Hoechst33242.

Technical Details

A stack of 7 confocal images 3.2 micrometers in thickness using a Zeiss LSM 510 confocal microscope with meta detector. The objective lens was a 63 x Plan Apo with NA: 1.4. The image represents a 'z' projection of the 7 0.75 micrometer thick slices

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
fibroblast of gingiva
Cellular Component
centriole
microtubule
nucleus
Attribution
Names
Alp Can
Link
Contributor's web site
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL43351
Archival Resource Key (ARK)
ark:/b7295/w9cil43351
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
Fluorescein (FITC)
Cy3
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1031px ——
Y 772px ——