Licensing

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Description

Representative time-lapse movie of a normal mouse mammary organoid in a 3D collagen I matrix. The cells are expressing td tomato as a membrane marker and eEGFP-actin using a keratin-14 promoter. Images were collected every 10 minutes. This movie is part of a group of movies that include CIL 42151-42168.

Biological Sources

NCBI Organism Classification
Mus musculus
(house mouse)
Cell Type
epithelial cell
Cellular Component
plasma membrane
actin cytoskeleton

Biological Context

Biological Process
dissemination
Mouse Gross Anatomy
mammary organoid

Attribution

Published
PNAS 2012 ; published ahead of print August 23, 2012
Other
Andrew Ewald

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
tdTomatoFP
EGFP
Processing History
contrast adjusted
confocal stack

Sample Preparation

Methods
living tissue
Relation To Intact Cell
mammary organoid

Dimensions

Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——
Time -- sec 110
*CIL – Cell Image Library accession number. Please use this to reference an image.