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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:41712*  Cite 
Description

High Voltage (1MeV) EM image of a metaphase chromosome isolated from CHO cells using a non-aqueous technique, squashed in 1:1 acetic acid:ethanol and transferred to an EM grid. A portion of a somewhat distorted chromatid pair occupies the center of the image. This micrograph was captured at a specimen tilt of 55 degrees. The accompanying image has a tilt of 50 degrees, creating a stereo pair that provides an oblique stereo view of the chromatids.

Technical Details

Cells were metaphase arrested with colcemid, exposed to a low ionic strength medium (0.07M phosphate buffer)containing 50% D20 as a microtubule stabilizer and chromosomes isolated by the non-aqueous technique of Stubblefield and Wray. Preparations were squashed in 3:1 acetic acid:ethanol,stained, and transferred to an EM grid. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources
NCBI Organism Classification
Cricetulus griseus
Cell Type
ovary
Cell Line
CHO
Cellular Component
nuclear chromosome
Biological Context
Biological Process
mitosis
mitotic metaphase
chromosome organization
Attribution
Names
Hans Ris
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL41712
Archival Resource Key (ARK)
ark:/b7295/w9cil41712
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
chemically fixed tissue
Relation To Intact Cell
squash
Dimensions
Spatial Axis Image Size Pixel Size
X 2744px 1nm
Y 4235px 1nm