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Gallery of transmission electron micrographs of ribosomes purified from a rat liver homogenate by sucrose gradient centrifugation, and negatively stained with uranyl acetate. The 3 columns at right include examples of large subunits with a round profile, the 2 center columns, 'skiff-shaped profiles, and the left column, rounded profiles from a glutaraldehyde fixed preparation.
Samples were placed on carbon coated collodion films, negatively stained with aqueous uranyl acetate, and examined with a Philips 300 TEM.
See also Plate IV in: N. Yoshiaki et al. 1971. Structure of liver ribosomes studied by negative staining. J Mol Biol 60:303-323
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George E. Palade
N. Yoshiaki et al. 1971. Structure of liver ribosomes studied by negative staining. J Mol Biol 60:303-323
George E. Palade EM Slide Collection
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