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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40672*  Cite 
Description

Stereo pair of an embedment-free section of a detergent-extracted unfertilized hamster egg. The central region of the egg contains two different types of fibrous networks: a thicker network of sheets that form small whorls and arcs and a thinner network. The sheets are specialized intermediate filament network in mammalian eggs and embryos (i.e., the cytoskeletal whorls) that are parallel bundles of intermediate filaments that disassemble into individual intermediate filaments. Scale bar is 1.0 µm.

Technical Details

Sections were imaged with a Phillips CM300 transmission electron microscope. See Capco DG, McGaughey RW. Dev Biol. 1986 Jun;115(2):446-58 for details on specimen processing. Figure 5 of this paper is a micrograph of a similarly prepared specimen.

Biological Sources
NCBI Organism Classification
Mesocricetus auratus
Cell Type
early embryonic cell
Cellular Component
intermediate filament cytoskeleton
Biological Context
Biological Process
intermediate filament cytoskeleton organization
Attribution
Names
James J. Faust
David G. Capco
OTHER
Arizona State University, Tempe, AZ
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40672
Archival Resource Key (ARK)
ark:/b7295/w9cil40672
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
detection of electrons
Source of Contrast
differences in adsorption or binding of stain
Processing History
stereo pair
Sample Preparation
Methods
detergent permeabilized
chemically fixed tissue
osmium tetroxide fixed tissue
embedment medium removal
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 787px ——
Y 377px ——