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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40663*  Cite 
Description

Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. This image shows branched actin filaments (cyan) at the neck–head junction. Dashed arrows indicate potential filament breakage. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel is available as CIL 40660. The uncolorized image is available as CIL 40659. Box 1, 2, and 3 (this image) are CIL 40661, 40662, and 40663, respectively.

Technical Details

Please refer to referenced article for details on cell extraction and processing for electron microscopy.

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
hippocampal neuron
Cellular Component
dendritic spine
dendritic shaft
actin cytoskeleton
Biological Context
Biological Process
dendritic spine organization
dendrite morphogenesis
Attribution
Names
Farida Korobova
Tatyana Svitkina
Published
Mol Biol Cell. 2010 Jan 1;21(1):165-76. Epub 2009 Nov 4.
Pubmed
19889835
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40663
Archival Resource Key (ARK)
ark:/b7295/w9cil40663
Grouping This image is part of a group.
Imaging
Image Type
grey scale graphic
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
Processing History
colorized
Dimensions
Spatial Axis Image Size Pixel Size
X 464px ——
Y 612px ——