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Description

The nascent phagosome membrane has a dense coat of acidosomes docked at its cytosolic surface well before the nascent vacuole pinches off the cytopharynx. This micrograph is a quick-freeze deep-etch image of a transverse section through the posterior portion of the buccal cavity with a nascent vacuole bulging from the cytopharynx. Living cells were spun down in a centrifuge and a slurry of cells was mounted on a copper support before slam freezing the living cells against a highly polished copper surface. Some paramecia had been disrupted and this cell was feeding on the particulate organelles of the disrupted cells. Thus the nascent vacuole contains these particles amassed against its luminal surface. The cilia in the buccal cavity obviously efficiently sweep the particles against the dorsal posterior surface. TEM taken on 5/26/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 4,000X. Bar = 1µm. Published in J. Cell Sci. 106:411-422, 1993. Adapted with permission.

Technical Details

The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
pre-autophagosomal structure membrane
vesicle membrane
oral apparatus

Biological Context

Biological Process
oral apparatus organization
membrane to membrane docking

Attribution

Name
Richard Allen (University of Hawaii)
Publisheds
J Cell Sci. 1993 Sep;106 ( Pt 1):411-22.
8270639
Link
http://www5.pbrc.hawaii.edu/allen/

Grouping

This image is part of a group.
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Dimensions

Spatial Axis Image Size Pixel Size
X 4000px 2nm
Y 3689px 2nm
*CIL – Cell Image Library accession number. Please use this to reference an image.