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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40501*  Cite 
Description

Enlargement of two mitochondria showing the cross sections of tubular cristae bear one pair of protrusions (these are the F1 subunits of F1F0 ATP synthase enzymes) while rows of protrusions can be viewed along some tangentially sectioned tubules. The tubules in some cases can be seen to arise from the inner membrane. TEM taken on 3/7/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 22,500X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
mitochondrion
mitochondrial membrane
Biological Context
Biological Process
mitochondrion ATP synthetase
Attribution
Names
Richard Allen (University of Hawaii)
Published
J Cell Biol. 1989 Jun;108(6):2233-40
Pubmed
2525561
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40501
Archival Resource Key (ARK)
ark:/b7295/w9cil40501
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
lead salt
osmium tetroxide
Processing History
recorded image
film
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 1819px 0.89nm
Y 1803px 0.89nm