Licensing
Description
Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibodies (shown in green), the cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue), (ii) anti-tubulin antibody as internal control and marker of microtubules, and (red), or (iii) calreticulin for the endoplasmic reticulum (ER) (yellow).
Technical Details
The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met: The publication and/or presentation are solely for informational and non-commercial purposes. The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.
Biological Context
- Biological Process
- organelle organization
- cytoskeleton organization
Attribution
- Names
- M Uhlen
- P Oksvold
- L Fagerberg
- E Lundberg
- K Jonasson
- M Forsberg
- M Zwahlen
- C Kampf
- K Wester
- S Hober
- H Wernerus
- L Björling
- F Ponten
- Published
- Nat Biotechnol. 2010 28(12):1248-50.
- Link
- Human Protein Atlas
- Pubmed
- 21139605
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- confocal microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of epitope
- Visualization Methods
- primary antibody plus labeled secondary antibody
- Processing History
- unprocessed raw data
- Data Qualifiers
- raw, unprocessed data
Sample Preparation
- Methods
- chemically fixed tissue
- permeabilized tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 1728px | —— |
| Y | 1728px | —— |