Licensing

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Description

Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibodies (shown in green), the cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue), (ii) anti-tubulin antibody as internal control and marker of microtubules, and (red), or (iii) calreticulin for the endoplasmic reticulum (ER) (yellow).

Technical Details

The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met: The publication and/or presentation are solely for informational and non-commercial purposes. The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.

Biological Context

Biological Process
organelle organization
cytoskeleton organization

Attribution

Names
M Uhlen
P Oksvold
L Fagerberg
E Lundberg
K Jonasson
M Forsberg
M Zwahlen
C Kampf
K Wester
S Hober
H Wernerus
L Björling
F Ponten
Published
Nat Biotechnol. 2010 28(12):1248-50.
Link
Human Protein Atlas
Pubmed
21139605

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data

Sample Preparation

Methods
chemically fixed tissue
permeabilized tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 1728px ——
Y 1728px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.