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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40416*  Cite 
Description

A transmission electron micrograph of a representative untreated chc1 mutant cell. CHC1 encodes clathrin heavy chain, which trimerizes to form triskelia that coat vesicles. chc1 mutant cells exhibit a vacuolar fragmentation phenotype. (8,000X). CIL 40415 is a sertraline treated chc1 mutant cell.

Technical Details

This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cell Type
chc1 mutant
Cellular Component
vacuole
Biological Context
Biological Process
organelle organization
vacuole fragmentation
Attribution
Names
Ethan Perlstein
Daniel Korostyshevsky
OTHER
Princeton EM Core Facility
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40416
Archival Resource Key (ARK)
ark:/b7295/w9cil40416
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
Processing History
unprocessed raw data
Sample Preparation
Methods
glutaraldehyde fixed tissue
tissue in plastic embedment
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 2504px ——
Y 2424px ——


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