A transmission electron micrograph of a representative untreated chc1 mutant cell. CHC1 encodes clathrin heavy chain, which trimerizes to form triskelia that coat vesicles. chc1 mutant cells exhibit a vacuolar fragmentation phenotype. (8,000X). CIL 40415 is a sertraline treated chc1 mutant cell.
This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.