Licensing

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Description

A transmission electron micrograph of a representative untreated chc1 mutant cell. CHC1 encodes clathrin heavy chain, which trimerizes to form triskelia that coat vesicles. chc1 mutant cells exhibit a vacuolar fragmentation phenotype. (8,000X). CIL 40415 is a sertraline treated chc1 mutant cell.

Technical Details

This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Biological Sources

NCBI Organism Classification
Saccharomyces cerevisiae
(Baker's Yeast, Brewer's Yeast)
Cell Type
chc1 mutant
Cellular Component
vacuole

Biological Context

Biological Process
organelle organization
vacuole fragmentation

Attribution

Names
Ethan Perlstein
Daniel Korostyshevsky
Other
Princeton EM Core Facility

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
Processing History
unprocessed raw data

Sample Preparation

Methods
glutaraldehyde fixed tissue
tissue in plastic embedment
Relation To Intact Cell
sectioned tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 2504px ——
Y 2424px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.