Time lapse movie of a porcine kidney LLCPK1 cell stably expressing photoactivable GFP-tubulin. Fluorescence was activated at metaphase in a vertical strip between the metaphase plate and spindle pole. The activated strip moves poleward, demonstrating a marked microtubule flux during mitotic metaphase. The time series of images used to prepare the movie is included in this group of images.
Cells were imaged using a Nikon Eclipse TE300 microscope with 100x phase NA 1.4 objective lens and spinning disk confocal head (Perkin Elmer) and images captured with a Hamamatsu Orca ER cooled CCD camera every 2 sec. Photoactivation was achieved with a 5-sec exposure to 413-nm light from an X-Cite 120 lamp. See also: NP Ferenz and P Wadsworth 2007. Prophase microtubule arrays undergo flux-like behavior in mammalian cells. Mol Biol Cell 18:3993-4002
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