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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40263*  Cite 
Description

Serial electron micrograph series through layers 1-4 of the primary visual cortex of the adult mouse. Reconstruction encompasses 450 X 350 X 52 um volume of tissue and contains ~ 1500 cell bodies. This same area of tissue was functionally characterized for orientation and direction selectivity using two photon calcium imaging. The reconstruction is also available at: http://openconnectomeproject.org/Bock11/ For more information see: Bock DD, Lee WC, Kerlin AM, Andermann ML, Hood G, Wetzel AW, Yurgenson S, Soucy ER, Kim HS, Reid RC. Network anatomy and in vivo physiology of visual cortical neurons. Nature. 2011 Mar 10;471(7337):177-82. PMID: 21390124. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

The animal was perfused transcardially (2% paraformaldehyde/2.5% glutaraldehyde). Seven 300 um thick coronal vibratome sections were cut. The vibratome sections were post-fixed and stained with 1% osmium tetroxide/1.5% potassium ferrocyanide followed by 1% uranyl acetate, dehydrated with a graded ethanol series, and embedded in resin (TAAB 812 Epon, Canemco). Serial thin (<50 nm) sections were cut on a Leica U6 ultramicrotome, picked up on pioloform-coated slot grids, and then post-stained with uranyl acetate and lead citrate. Serial sections were imaged with a JEOL 1200EX, magnification, 2000.0 X, accelerating voltage, 120.0 kv, and compiled into a composite image.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
pyramidal cell
stellate cell
astrocyte of the cerebral cortex
Cellular Component
cell body
neuronal cell body
Attribution
Names
R. Clay Reid
Link
CCDB 8448
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40263
Archival Resource Key (ARK)
ark:/b7295/w9cil40263
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Visualization Methods
uranyl salt
Data Qualifiers
processed data
Sample Preparation
Methods
osmium tetroxide fixed tissue
glutaraldehyde fixed tissue
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
X 453px ——


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