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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40241*  Cite 

Large scale mosaic of a parasagittal section of rat brain stained with an antibody against a plasma membrane calcium-dependent ATPase, PMCA1a (shown here in red), and counter stained with Hoescht 33342 to reveal cell nuclei (shown here in green). This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information see: Kenyon et al.(2010) J Comp Neurol. 518(16):3169-83, PMID 20575074.

Technical Details

Rat brain was perfused with 4% paraformaldehyde and sectionedrain section was immunolabeled for PMCA1a using CR1a antibody from Emanuel Strehler (1:2000). Secondary antibody was conjugated to Alexa Fluor 568. Section was then counterstained with Hoescht 33342 (Invitrogen) to visualized all cell nuclei. The sections were coverslipped in Gelvatol. Images were acquired using a BioRad RTS2000 with a Nikon 60X N.A. 1.4 oil-immersion objective. 46 x 19 maximum projection images were tiled together to generate this composite image.

Biological Sources
NCBI Organism Classification
Rattus norvegicus
Cell Type
CNS neuron (sensu Vertebrata)
hippocampal neuron
Cellular Component
Alain Burette
Emanuel E. Strehler
Richard J. Weinberg
Katherine A. Kenyon
CCDB 7407
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Sample Preparation
formaldehyde fixed tissue
Relation To Intact Cell
vibratome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 512px ——
Y 201px ——