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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40187*  Cite 
Description

Single computed slice through a double tilt electron tomogram of a lamellar structure observed in HeLa cells transfected with mutant connexin 50, imaged using intermediate voltage electron microscopy, and labeled using the tetracysteine-ReHAsH system and fluorescence photoooxidation. The concentric double membrane layers of the aggregate appear to form "onion skin" sheets that do not form a closed structure along the long axis. The differential staining between the outside and inside is most likely due to impeded diffusion of DAB into the interior of these accumulations. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information see: Lichtenstein et al. The cytoplasmic accumulations of the cataract-associated mutant, Connexin50P88S, are long-lived and form in the endoplasmic reticulum Exp Eye Res. 2009 Mar;88(3):600-9. PMID: 19073179

Technical Details

ReAsH-labeled cells were fixed in 2% glutaraldehyde in 0.1 M sodium cacodylate, pH 7.4 at 4°C. After 20 min, the cells were rinsed in 0.1 M cacodylate pH 7.4 and incubated for 30-45 min with blocking buffer (10 mM KCN, 10 mM aminotriazole, 0.01% hydrogen peroxide/50 mM glycine in 0.1 M cacodylate pH 7.4). This buffer was then replaced with 1 mg/ml diaminobenzidine (DAB) in blocking buffer, and photoconversion was performed using intense illumination (75 W xenon arc lamp without neutral density filters) focused through the microscope objective. Rinsing and further preparation of the sample for transmission electron microscopy were performed as described in Gaietta et al. (2002). Sections were stained with uranyl acetate prior to acquiring tilt images.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Line
HeLa
Cellular Component
connexon complex
plasma membrane
Attribution
Names
Eric Beyer
Link
CCDB 6348
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40187
Archival Resource Key (ARK)
ark:/b7295/w9cil40187
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
Parameters Imaged
electron density
Data Qualifiers
processed data
Sample Preparation
Methods
osmium tetroxide fixed tissue
glutaraldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
X 512px ——