Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40113^* Cite

Description

Electron micrograph of a cultured Drosophila DL1 cell infected with flock house virus, prepared using chemical fixation followed by routine embedding for electron microscopy. This specimen was prepared as a control to compare different high pressure freezing protocols. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Cell pellets were conventionally prepared for electron microscopy by incubation in 2% glutaraldehyde in 100 mM cacodylate approx 5 min) and then on ice for 30 minutes, followed by 1 percent osmium tetroxide in double distilled water for 1 hour and 2 percent UA in water overnight. Images were acquired using a JEOL4000EX IVEM, magnification: 30000.0; accelerating voltage: 80.0 keV.

Biological Sources

NCBI Organism Classification: Drosophila melanogaster
Cell Type: embryonic cell
Cellular Component: cell body

Attribution

Names: Mark Ellisman; Gina Sosinsky; Ying Jones
Link: CCDB 3937

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40113
Archival Resource Key (ARK): ark:/b7295/w9cil40113

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode
Parameters Imaged: electron density
Data Qualifiers: processed data

Sample Preparation

Methods: osmium tetroxide fixed tissue; glutaraldehyde fixed tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	512px	——
Y	512px	——