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Licensing
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39992*  Cite 
Description

A single computed slice from a tomographic reconstruction of a lucifer yellow injected spiny dendrite from a striatal medium spiny neuron from the neostriatum of a dopamine transporter knock out mouse. This reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Tissue from mouse strain: B6;129-/Slc6a3 tm2Mca, dopamine transporter knockout. Individual cells in fixed tissue were injected with Lucifer yellow and stored in 4% paraformaldehyde at 4° C. Fluorescence was photoconverted using DAB and a confocal laser light source. After this process, tissue was washed thoroughly in PBS and processed for EM by osmication, dehydration, and embedding in epoxy resin. For detailed methods, see the protocol provided in the CCDB. The tomogram was generated using an Hitachi UHVEM. Single tilt images spanned -70 to 70° in 2° increments. Magnification, 3000X, accelerating voltage, 3.0 MeV.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
CNS neuron (sensu Vertebrata)
Cellular Component
dendritic spine
dendrite
Attribution
Names
Diana Price
Aki Laakso
Michele Cyr
Maryann Martone
Naoko Yamada
Andrea Thor
Monica Berlanga
Link
CCDB 3561
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39992
Archival Resource Key (ARK)
ark:/b7295/w9cil39992
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
FBbi:00000622
detection of electrons
illumination by electrons
Parameters Imaged
electron density
Visualization Methods
intracellular filling
lucifer yellow
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——