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Description

PALM images of two regions of Drosophila metaphase chromosomes with red arrowheads pointing to filamentous structures. Overview images of the chromosome clusters are seen the the grouped image (CIL:39747) See Fig 3 in: A Matsuda et al. (2010) Condensed mitotic chromosme structure at nanometer resolution using PALM and EGFP-histones. PLOSone 5:e12768.

Technical Details

PALM was achieved using a custom wide-field microscope platform with 100x NA 1.4 objective lens or 150X TIRF NA 1.45 lens, yielding a pixel size at the CCD camera of 0.0792 or 0.0528 micrometers respectively. See also: A Matsuda et al. (2010) Condensed mitotic chromosme structure at nanometer resolution using PALM and EGFP-histones. PLOSone 5:e12768.

Biological Sources

NCBI Organism Classification
Drosophila melanogaster
(Common fruit fly)
Cell Type
early embryonic cell
Cellular Component
nuclear chromosome
chromatin

Biological Context

Biological Process
DNA packaging
chromatin organization

Attribution

Names
A Matsuda
L Shao
J Boulanger
C Kervrann
PM Carlton
P Kner
D Agard
J Sedat
Published
A Matsuda et al. (2010) Condensed mitotic chromosme structure at nanometer resolution using PALM and EGFP-histones. PLOSone 5:e12768
Link
PLOSone
Pubmed
20856676

Grouping

This image is part of a group.

Imaging

Image Type
portrayed image
Imaging Mode
PALM (photoactivation localization microscopy)
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
4',6-diamidino-2-phenylindole (DAPI)
Processing History
PALM reconstruction
Data Qualifiers
processed data

Sample Preparation

Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 942px ——
Y 403px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.