Licensing

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Description

EM images of chromatin fibers reconstituted from tandem arrays of a strong nucleosome positioning sequence flanked by 'linker' DNA, creating fibers containing 22 nucleosomes and a nucleosome repeat length (NRL) of 177 bp. Reconstitutes had saturating amounts of purified core histones and linker histones. (A) shows a montage of 'end on' views of fibers obtained by cryo-fixation and recorded in the frozen hydrated state. (B and C) contain views of 3D models considered to correspond to the images at left. See Fig 1 in P.J. Robinson et al. 2006. EM measurements define the dimensions of the "30-nm" chromatin fiber: Evidence for a compact, interdigitated structure. Proc Natl Acad Sci USA 103:6506-6511.

Technical Details

See: P.J. Robinson et al. 2006. EM measurements define the dimensions of the "30-nm" chromatin fiber: Evidence for a compact, interdigitated structure. Proc Natl Acad Sci USA 103:6506-6511.

Biological Sources

Cellular Component
chromatin

Biological Context

Biological Process
chromatin organization
DNA organization

Attribution

Names
Philip J Robinson
Louise Fairall
Van A Huynh
Daniela Rhodes
Published
P.J. Robinson et al. 2006. EM measurements define the dimensions of the "30-nm" chromatin fiber: Evidence for a compact, interdigitated structure Proc Natl Acad Sci USA 103:6506-6511
Link
Proceedings National Academy of scienc...
Pubmed
16617109

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
portrayed image
Imaging Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
intrinsic mass distribution
Visualization Methods
visualization of contiguous regions
Processing History
montage
Data Qualifiers
processed data

Sample Preparation

Methods
cryofixed tissue
Relation To Intact Cell
isolated subcellular component

Dimensions

Spatial Axis Image Size Pixel Size
X 514px ——
Y 366px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.