Licensing

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Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cln1. Cln 1 is a cyclin-dependent protein kinase regulator. These phase and GFP z-series images are part of an image group containing CIL 39601-39605. Note that there are additional groups of images of other cell cycle regulator proteins by the same authors available in the Library.

Technical Details

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please read the microscopy section of the referenced paper for details before image analysis.

Biological Sources

NCBI Organism Classification
Saccharomyces cerevisiae
(Baker's Yeast, Brewer's Yeast)
Cellular Component
CLN1 protein
nucleus
cytoplasm

Attribution

Names
David Ball
Jean Peccoud
Published
PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed
22046265

Grouping

This image is representative of a group of similar images.

Sample Preparation

Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 512px 213.4nm
Y 512px 213.4nm
Z 21 0.5μm
Time 300 sec 61
*CIL – Cell Image Library accession number. Please use this to reference an image.