A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged TEM1. TEM1 is a GTP-binding protein of the ras superfamily involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis. These phase, gfp z-series images are part of an image group that ranges from CIL:39591-39595. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.
Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.
- David Ball
- Jean Peccoud
- PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
GroupingThis image is representative of a group of similar images.
- Image Type
- recorded image
- Imaging Mode
- fluorescence microscopy
- phase contrast microscopy
- Parameters Imaged
- fluorescence emission
- elastic scattering of electrons
- Source of Contrast
- distribution of a specific protein
- differences in amount of elastic light scattering
- Visualization Methods
- visualization of contiguous regions
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