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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39588*  Cite 
Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged SWI5. SWI5 is a transcription factor that recruits the Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; is required for expression of the HO gene controlling mating type switching; and whose localization to the nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase. These phase, gfp z-series images are part of an image group that ranges from CIL:39586-39590. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

Technical Details

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cellular Component
SWI5 protein
nucleus
cytoplasm
Attribution
Names
David Ball
Jean Peccoud
Published
PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed
22046265
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39588
Archival Resource Key (ARK)
ark:/b7295/w9cil39588
Grouping This image is part of a group.
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 213.4nm
Y 512px 213.4nm
Time 300 microns 61
Time 0.5 microns 21