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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39561*  Cite 
Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP-tagged MCM1. MCM1 is a transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes These phase, gfp z-series images are part of an image group that ranges from CIL:39561-39565. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

Technical Details

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cellular Component
MCM1 protein
nuclear chromatin
nucleus
Attribution
Names
David Ball
Jean Peccoud
Published
PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed
22046265
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39561
Archival Resource Key (ARK)
ark:/b7295/w9cil39561
Grouping This image is part of a group.
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 213.4nm
Y 512px 213.4µm
Z 21px 0.5µm
Time 300 seconds 61


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