Licensing

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Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged Cln2. Cln2 is a G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p). These phase, gfp z-series images are part of an image group that ranges from CIL:39526-39530. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

Technical Details

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.

Biological Sources

NCBI Organism Classification
Saccharomyces cerevisiae
(Baker's Yeast, Brewer's Yeast)
Cellular Component
CLN2 Protein
cyclin-dependent protein kinase holoenzyme complex

Attribution

Names
David Ball
Jean Peccoud
Published
PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed
22046265

Grouping

This image is representative of a group of similar images.

Sample Preparation

Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 512px 213.4nm
Y 512px 213.4μm
Z 21 0.5μm
Time 300 sec 61
*CIL – Cell Image Library accession number. Please use this to reference an image.