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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:39335*  Cite 
Description

A contractile vacuole in systole. The CV has collapsed into vesicles and tubules. Three CV pores can be seen where the CV meets the pellicle. Smooth membrane cisterna surrounds some cytoplasm, possibly a developing stage of an autophagic vacuole. TEM taken on 6/12/69 by R. Allen with Philips 300 operating at 60kV. Neg. 9,250X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness.

Biological Sources
NCBI Organism Classification
Opercularia [NCBITaxon:168247]
Opercularia coarctata
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, # LB1657/1
Cellular Component
macronucleus
oral apparatus
contractile vacuole
cell cortex
cytoplasm
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL39335
Archival Resource Key (ARK)
ark:/b7295/w9cil39335
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 3195px 1.1nm
Y 4000px 1.1nm