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Description

Leaves from wildtype Arabidopsis thaliana were fixed, embedded, sectioned, reacted with antibodies to the FtsZ1-1 protein (upper panels) or FtsZ-2 (lower panels), prokaryotic cell division proteins that are a structural homolog of tubulin. The protein is localized at the site of division which occurs by constricting at the chloroplast mid-point. Shown (from left to right) are focal planes from the bottom, middle and top of the chloroplast, with the projection image at far right revealing the ring-shaped FtsZ distribution.

Technical Details

Leaves were fixed in FAA (formalin acetic acid), embedded in low melting point polyester wax, and 7 micrometer sections cut and attached to poly-L-lysine slides. Sections were de-waxed, treated with an antigen retrieval procedure, and processed for immunofluorescence using incubation with an affinity purified polyclonal antibody, followed by FITC-conjugated secondary antibody. Slides were viewed with an Olympus BH2 microscope equipped with DIC optics. Images were recorded on film, or a video camera (Optronics DEI 750 using a 100x 1.25 NA objective lens. Shown are 3 focal planes. To generate the 3D image shown at far right the z-stack was rotated 30 degrees and projected. See S. Vitha et al. 2001 J Cell Biol 153:111-119.

Biological Sources

NCBI Organism Classification
Arabidopsis thaliana
(thale cress)
Cell Type
leaf
Cellular Component
chloroplast
plastid part

Biological Context

Biological Process
chloroplast organization
plastid organization

Attribution

Names
Stanislav Vitha
Rosemary S McAndrew
Katherine W Osteryoung
Published
S Vitha et al. 2001 FtsZ ring formation at the chloroplast division site in plants. J Cell Biol 153:111-119
Pubmed
11285278

Grouping

This image is representative of a group of similar images.

Imaging

Image Type
recorded image
Imaging Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
Fluorescein (FITC)
Data Qualifiers
processed data

Sample Preparation

Methods
formaldehyde fixed tissue
tissue in wax embedment
Relation To Intact Cell
microtome-sectioned tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 342px ——
Y 184px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.