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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:38818*  Cite 
Description

Dorsal root ganglion cells from embryonic mice were cultured and stimulated to produce cyclooxygenase-2 (COX-2). Cultures were immuno-stained for neuron-associated tubulin (green), COX-2 (red) and DNA (blue). COX-2 is primarily expressed in neurons.

Technical Details

After induction of COX-2, cultures were fixed with paraformaldehyde overnight, then immunostained with a mouse antibody to neuronal class III beta-tubulin followed by an Alexa 488-conjugated goat anti-mouse to identify neurons. COX-2 was imaged using Fluorocoxib A, a selected COX-2 inhibitor conjugated to carboxy-X-rhodamine (see http://cancerres.aacrjournals.org/content/70/9/3618.full). Preparations were counterstained with DAPI, and imaged with a Leica CMS microscope using a 40x 0.8 NA objective lens. Images were recorded with a Qimaging Qi CCD camera.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
dorsal root ganglion
neuron of the dorsal spinal cord
Attribution
Names
Kelsey C Duggan
Daniel J Hermanson
Joel Musee
Jeffery J Prusiakiewicz
Jami L Scheib
Bruce D Carter
Surajit Banerjee
J A Oates
Lawrence J Marnett
Published
NIGMS Biomedial Beat
Published
K C Duggan et al. 2011 (R)-Profens are substrate-selective inhibitors of endocannabinoid oxygenation by COX-2 Nat
Pubmed
22053353
Link
NIGMS Biomedical Beat
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL38818
Archival Resource Key (ARK)
ark:/b7295/w9cil38818
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1392px 0.24µm
Y 1040px 0.24µm