Licensing

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Description

This differential interference contrast image (DIC) corresponds to the same image field as the diffraction limited TIRF image CIL 38599 and the 2-color photoactivation localization microscopy (PALM) image 38598. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 2 in PNAS U S A. 2007 Dec 18;104(51):20308-13.

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Type
fibroblast
Cell Line
foreskin fibroblast
Cellular Component
cell

Attribution

Names
Hari Shroff
Catherine G Galbraith
James A. Galbraith
Helen White
Jennifer Gillette
Scott Olenych
Michael W. Davidson
Eric Betzig
Published
Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20308-13. Epub 2007 Dec 12. Erratum in: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):15220.
Link
article
Pubmed
18077327

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
differential interference contrast microscopy
Parameters Imaged
optical path length gradient
Source of Contrast
boundaries between regions with different refractive index
Visualization Methods
visualization of contiguous regions
Processing History
smoothing filter
Data Qualifiers
processed data

Sample Preparation

Methods
formaldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 850px ——
Y 714px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.