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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:37154*  Cite 
Description

This is Video S1 and it corresponds to Figure 2A. It shows that local blebbistatin micropipetting, but not DMSO, increases spine formation and extension (arrowheads). Equal volumes of blebbistatin and DMSO were used. Images were acquired every ~6 seconds using confocal microscopy. 20 frames/sec shown. A DIV 7 cortical neuron expressing DsRed2 was locally micropipetted with either DMSO or 100 mM blebbistatin at the indicated times. Note the increase in the fraction of spines that appear and extend in response to blebbistatin. Arrowheads indicate either nascent or elongating spines.

Technical Details

Confocal images were collected on an Olympus Fluoview 1000 microscope (IX81 base) equipped with a 60X/1.35 NA (oil)UPLSAPO 60X objective (Olympus). DsRed was excited with the 543 nm laser line of a He-Ne laser. Fluorescence emission was collected using the following dichroic mirror/filter: SDM640/BA560–620.

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
CNS neuron (sensu Vertebrata)
Cellular Component
dendritic spine
dendrite
dendritic shaft
cytoplasm
Attribution
Names
Jennifer L. Hodges
Karen Newell-Litwa
Hannelore Asmussen
Miguel Vicente-Manzanares
Alan Rick Horwitz
Link
http://www.plosone.org/article/info%3Adoi%2F1...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL37154
Archival Resource Key (ARK)
ark:/b7295/w9cil37154
Grouping This image is part of a group.
Imaging
Image Type
time lapse microscopy
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
DsRed
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 192px 0.38µm
Y 192px 0.38µm
Time 6 seconds 500