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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36818*  Cite 
Description

Image is of a section of a liver from a CD-1 mouse treated intravenously with siRNA-Cy5 enapsulated with lipid nanoparticle (LNP201-siRNA-Cy5) for 2 hours. siRNA-Cy5 (red) can be observed in the sinusoids and inside hepatocytes. Cells are outlined with phalloidin-Alexa488 (green) and nuclei are stained with DAPI (blue). The black section in the lower right corner is a vein of the liver. This image is Fig 2F of J Histochem Cytochem 2011 59: 727 and Cover Image for the Journal of Histochemistry and Cytochemistry, August 2011.

Technical Details

7 micron thick cryosection of liver was fixed with 4% paraformaldehyde in PBS for 10 min. Sections were stained with phalloidin-Alexa 488 and DAPI. Images were captured with an Olympus BX61 microscope with a 1.4 mega-pixel progressive scan camera.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
hepatocyte
sinusoids
Cellular Component
filamentous actin
nucleus
Attribution
Names
Bin Shi
Ed Keough
Andrea Matter
Karen Leander
Stephanie Young
Ed Carlini
Alan B. Sachs
Weikang Tao
Marc Abrams
Bonnie Howell
Laura Sepp-Lorenzino
Published
J Histochem Cytochem 2011 59: 727
Pubmed
21804077
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36818
Archival Resource Key (ARK)
ark:/b7295/w9cil36818
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
cryostat-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1344px ——
Y 1024px ——


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