The Cell: An Image Library - Image CIL:36750

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CIL:36750^*

Description

Quick-freeze deep-etch image of a DV-III containing paracrystalline sheets in its lumen. The sheets have parallel rows of subunits spaced at 20nm center-to-center but the chemical nature of the sheets is unknown; they do not appear to be acid phosphatase positive. TEM taken on 6/7/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 24,800X. Bar = 0.2µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: food vacuole; vesicle lumen

Biological Context

Biological Process: digestive system process

Attribution

Names: Richard Allen (University of Hawaii); C. Schroeder
Link: http://www5.pbrc.hawaii.edu/allen/

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Imaging Mode: electron density
Parameters Imaged: elastic scattering of electrons; electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: transmission electron microscopy (TEM)
Processing History: freeze_fracture/freeze_etch; shadowing and plating; recorded image; film; Print of negative scanned for Photoshop

Data Qualifiers: processed data

Sample Preparation

Methods: freeze_fracture/freeze_etch
Relation To Intact Cell: shadowing and plating; freeze_fracture/freeze_etch; isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	2124px	——
Y	2622px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.