As with acidosome fusion with the DV-I, lysosome fusion with the DV-II also seems to occur nearly all at once resulting in a phagolysosome (DV-III) with a highly irregular surface. However, unlike the transformation of DV-I into DV-II where the DV-I membrane is removed by immunogold-labeled tubules we have very little immunological evidence that the DV-II membrane is removed during the transition from phagoacidosome to phagolysosome. Although there is some morphological indication for tubular and vesicular formation around these early phagolysosomes that might indicate a similar transformation. The vacuole contents seen here are thought to be the lipid micelles of the axenic medium. TEM taken on 2/27/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 7,000X. Bar = 1µm.
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
|Spatial Axis||Image Size||Pixel Size|