The Cell: An Image Library - Image CIL:36644

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CIL:36644^*

Description

Large patches of rough ER surrounded by Golgi stacks (visible only at high magnification) have formed in the cytosol along with fields of lipid bodies. By day 2 these ER and lipid fields will be dispersed or depleted. For culture age study see Fok and Allen, Eur. J. Cell Biol. 25:182-193, 1981. TEM taken on 4/28/80 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 4,050X. Bar = 1µm.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Paramecium multimicronucleatum
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cellular Component: endoplasmic reticulum; Golgi stack

Biological Context

Biological Process: endoplasmic reticulum organization; Golgi organization

Attribution

Name: Richard Allen (University of Hawaii)
Link: http://www5.pbrc.hawaii.edu/allen/

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Imaging Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; uranyl salt; lead salt; osmium tetroxide
Processing History: recorded image; film; Print from negative scanned for Photoshop.

Data Qualifiers: processed data

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3129px	——
Y	3378px	——

*CIL – Cell Image Library accession number. Please use this to reference an image.