Basal body formation occurs most prominently in the equatorial region of the predivision cell. A new basal body begins to develop perpendicular to the mature basal body at its proximal anterior surface. The basal body lengthens and tilts toward the surface. At first no new accessory microtubular ribbons or kinetodesmal fibers are observed, only those accessories associated with the mature basal bodies are present. TEM taken on 8/14/67 by R. Allen with Philips 200 operating at 60kV. Neg. 19,200X. Bar = 1µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. There is a high resolution version of this image in the library (CIL:39796) which is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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