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Description

The first image in this multi-image tiff file is a stimulated emission depletion image (STED) of microtubules in a Drosophila S2 cell. The second image is the corresponding diffraction limited image obtained with conventional fluorescence microscopy. Note the increase in spatial resolution in the STED image compared to the diffraction limited image. Images were collected as part of the Marine Biological Laboratory Neurobiology Course, Summer 2011.

Technical Details

Cells were plated on Con-A coated coverslips, chemically fixed, and labeled with an anti-tubulin primary antibody and an Alexa Fluor 488 secondary antibody. Images were collected on a Leica STED microscope.

Biological Sources

NCBI Organism Classification
Drosophila melanogaster
(Common fruit fly)
Cell Type
epithelial cell
Cell Line
Schneider S2
Cellular Component
microtubule

Biological Context

Biological Process
microtubule cytoskeleton organization

Attribution

Names
Victor Cazares
Luke Chao
Till Andlauer
James A Galbraith
Catherine G Galbraith011
Link
MBL Neurobiology Course 2011
Other
MBL Neurobiology Course 2011

Imaging

Image Type
recorded image
Imaging Mode
stimulated emission depletion (STED)
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
primary antibody plus labeled secondary antibody
Alexa Fluor 488
Processing History
maximum projection

Sample Preparation

Methods
formaldehyde fixed tissue
glutaraldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 1269px 0.0252μm
Y 1197px 0.0252μm
*CIL – Cell Image Library accession number. Please use this to reference an image.