The first image in this multi-image tiff file is a stimulated emission depletion image (STED) of microtubules in a Drosophila S2 cell. The second image is the corresponding diffraction limited image obtained with conventional fluorescence microscopy. Note the increase in spatial resolution in the STED image compared to the diffraction limited image. Images were collected as part of the Marine Biological Laboratory Neurobiology Course, Summer 2011.
Cells were plated on Con-A coated coverslips, chemically fixed, and labeled with an anti-tubulin primary antibody and an Alexa Fluor 488 secondary antibody. Images were collected on a Leica STED microscope.