Alternate header for print version


Licensing
Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
tweet  
Comments: Powered by Pnyxe
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:36147*  Cite 
Description

The first image in this multi-image tiff file is a structured illumination image (SIM) of microtubules in a Drosophila S2 cell. The second image is the corresponding diffraction limited image obtained with conventional fluorescence microscopy. Note the two-fold increase in spatial resolution in the SIM image compared to the diffraction limited image. The z-series are available as CIL 36797. Images were collected as part of the Marine Biological Laboratory Neurobiology Course, Summer 2011.

Technical Details

Cells were plated on Con-A coated coverslips, chemically fixed, and labeled with an anti-tubulin primary antibody and an Alexa Fluor 488 secondary antibody. Images were collected on a Zeiss Elyra Super-resolution microscope by Chris Rieken

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
epithelial cell
Cell Line
Schneider S2
Cellular Component
microtubule
Biological Context
Biological Process
microtubule cytoskeleton organization
Attribution
Names
Victor Cazares
Luke Chao
James A Galbraith
Catherine G Galbraith
Link
MBL Neurobiology Course 2011
OTHER
MBL Neurobiology Course 2011
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL36147
Archival Resource Key (ARK)
ark:/b7295/w9cil36147
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
structured illumination microscopy (SIM)
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
primary antibody plus labeled secondary antibody
Alexa Fluor 488
Processing History
maximum projection
Sample Preparation
Methods
formaldehyde fixed tissue
glutaraldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1054px 0.0397µm
Y 1028px 0.0397µm


Ascb_logo
Ccdb_logo_banner
Nif_button2
Dci_button
Labbyrcw