The first image in this multi-image tiff file is a structured illumination image (SIM) of microtubules in a Drosophila S2 cell. The second image is the corresponding diffraction limited image obtained with conventional fluorescence microscopy. Note the two-fold increase in spatial resolution in the SIM image compared to the diffraction limited image. The z-series are available as CIL 36797. Images were collected as part of the Marine Biological Laboratory Neurobiology Course, Summer 2011.
Cells were plated on Con-A coated coverslips, chemically fixed, and labeled with an anti-tubulin primary antibody and an Alexa Fluor 488 secondary antibody. Images were collected on a Zeiss Elyra Super-resolution microscope by Chris Rieken