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Description

A time lapse experiment of Saccharomyces cerevisiae expressing GFP tagged MAD2. MAD2 is a non-essential gene that encodes a component of the spindle checkpoint. The spindle checkpoint delays the onset of anaphase in cells with defects in mitotic spindle assembly or in the attachment of chromosomes to the spindle microtubules. The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators. Like other spindle checkpoint mutants, mad2 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization. Mad2p forms a tight complex with another spindle checkpoint protein, Mad1p, throughout the cell cycle. Mad2p also forms a complex with Cdc20p, which activates the anaphase promoting complex, and Mad3p; the presence of Mad1p is required for the complex to form. Bub1p, Bub3p, Mad1p, Mad2p, Mad3p, and the protein kinase Mps1p act in a branch of the spindle checkpoint pathway that may prevent premature chromosome disjunction. A second branch involves Bub2p and Bfa1p, and may prevent cytokinesis prior to chromosome segregation. Homologs of Mad2p act in the spindle checkpoint in Xenopus (Xmad2) and human (MAD2L1 and MAD2L2. MAD2 was originally identified as the open reading frame YJL031C. However, it is now known that MAD2 corresponds to the adjacent open reading frame YJL030W, while YJL031C encodes the geranylgeranyltransferase subunit Bet4p. These phase, gfp images are part of an image group that ranges from CIL:35680-35699. Note that there are additional groups showing time series of other cell cycle regulation proteins by the same authors in the Library.

Technical Details

Time-lapse images were collected on a DeltaVision system (Olympus IX71) with 60x/1.42 NA objective at 5 minute intervals. Please see the microscopy section in the referenced manuscript for details for image analysis.

Biological Sources

NCBI Organism Classification
Saccharomyces cerevisiae
(Baker's Yeast, Brewer's Yeast)
Cellular Component
MAD2 protein
mitotic checkpoint complex
nuclear pore

Attribution

Names
David Ball
Jean Peccoud
Published
PLoS One. 2011;6(10):e26272. Epub 2011 Oct 26.
Pubmed
22046265

Grouping

This image is representative of a group of similar images.

Sample Preparation

Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 512px 213.4nm
Y 512px 213.4nm
Time 300 sec 61
*CIL – Cell Image Library accession number. Please use this to reference an image.