Licensing

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Description

Time lapse movie of mitosis in Xenopus laevis tissue culture cell. The sequence illustrates the requirement for all chromosomes to be aligned on the metaphase plate before anaphase is initiated.

Technical Details

Phase contrast images were captured at 15 sec intervals using a Zeiss 200 inverted microscope with 63x 1.4NA objective lens.

Biological Sources

NCBI Organism Classification
Xenopus laevis
(African clawed frog)
Cell Type
tissue culture cell
Cell Line
S3
Cellular Component
nucleus
nuclear chromosome
spindle

Biological Context

Biological Process
mitosis
chromosome organization
nucleus organization

Attribution

Names
John R. Daum
Gary J Gorbsky

Sample Preparation

Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 518px ——
Y 396px ——
Time 15 sec 55
*CIL – Cell Image Library accession number. Please use this to reference an image.