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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35546*  Cite 
Description

CHO cells were arrested in metaphase with colcemid, swollen in 75mM KCl, fixed, disrupted, and observed using High Voltage EM. This image was recorded with a specimen tilt angle of 46 degrees. Grouped with it is an image of the same area at a tilt angle of 54 degrees, providing an oblique stereo view of chromosome organization.

Technical Details

Metaphase-arrested cells were fixed with 3:1 ethanol:acetic acid, transferred to 50% acetic acid, and passed though a syringe to break cells. Drops of sample were placed on formvar coated grids, stained with uranly acetate, and critical point dried. Grids were observed with the Wisconsin HVEM at 1 MeV. See Ris (1981) Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth. Cell Biol 18:229-246.

Biological Sources
NCBI Organism Classification
Cricetulus griseus
Cell Type
ovary
Cell Line
CHO
Cellular Component
nuclear chromosome
Biological Context
Biological Process
mitosis
Attribution
Names
Hans Ris
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35546
Archival Resource Key (ARK)
ark:/b7295/w9cil35546
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data
Sample Preparation
Methods
coagulating-fixative fixed tissue
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 4774px 1.5nm
Y 2990px 1.5nm