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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35452*  Cite 

Low magnification of whole polytene chromosome from 3rd instar larva of Drosophila melanogaster imaged with high voltage EM at 1MeV showing the characteristic pattern of bands and interbands.

Technical Details

Salivary gland cells were squashed in 45% acetic acid, slides frozen in liquid nitrogen, cover slips removed, stained with uranyl magnesium acetate, dehydrated with ethanol, transferred to formvar coated grids, critical point dried, and imaged at 1MeV with the Wisconsin HVEM. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic microscopy of chromosomes. Meth Cell Biol 22:77-96.

Biological Sources
NCBI Organism Classification
Drosophila melanocephala
Cell Type
salivary gland
Cellular Component
polytene chromosome
Hans Ris
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
Image Mode
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data
Sample Preparation
coagulating-fixative fixed tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 3750px 2.3µm
Y 6941px 2.3µm