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Description

High Voltage (1MeV) TEM image of a 'thick' section of polytene chromosome from salivary gland of 3rd instar larva of Drosophila melanogaster showing the band-interband organization. This image was captured with a specimen tilt of 56 degrees. Grouped with it is an image of the same region taken at a tilt angle of 45 degrees, providing an oblique stereo view of the structure.

Technical Details

Salivary glands were dissected, fixed with paraformaldehyde, post-fixed with osmium tetroxide, and embedded in plastic. 4 micron sections were cut, stained with aqueous uranyl magnesium acetate, and observed with the Wisconsin HVEM at 1 MeV. See Ris, H 1981 Stereoscopic Electron Microscopy of chromosomes. Meth Cell Biol 22:77-96; Ris, H 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246

Biological Sources

NCBI Organism Classification
Drosophila melanogaster
(Common fruit fly)
Cell Type
salivary gland
Cellular Component
polytene chromosome

Biological Context

Biological Process
chromosome organization

Attribution

Name
Hans Ris, University of Wisconsin, Madison, WI

Grouping

This image is part of a group.

Imaging

Image Type
film
Imaging Mode
FBbi:00000622
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data

Sample Preparation

Methods
chemically fixed tissue
paraformaldehyde fixation
osmium tetroxide fixed tissue
tissue in plastic embedment
Relation To Intact Cell
microtome-sectioned tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 5580px 1.5nm
Y 3690px 1.5nm
*CIL – Cell Image Library accession number. Please use this to reference an image.