Licensing

Pd
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
Comments: Powered by Pnyxe

Description

Whole human female metaphase chromosome from metaphase arrested cell swollen in hypotonic medium and imaged at 1 MeV. This image was taken with a specimen tilt of 55 degrees. Grouped with it is an image of the same area at 45 degrees tilt, providing an oblique stereo view of the chromosome.

Technical Details

HeLa cells were metaphase-arrested by a 3hr treatment with colcemid, the cells mechanically broken by passage through a 22-gauge needle and exposed to hypotonic 75 mM KCl for 10 min. Samples were fixed with 3:1 methanol:acetic acid, squashed in 45% acetic acid, transferred to a formvar coated grid, critical point dried, and imaged with the Wisconsin HVEM at 1 MeV. See Ris (1978) Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:229-246; Ris (1981) Stereoscopic microscopy of chromosomes. Meth Cell Biol 22:77-96.

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Type
cevical carcinoma
Cell Line
HeLa
Cellular Component
condensed chromosome

Biological Context

Biological Process
nuclear division
chromosome organization
mitosis

Attribution

Name
Hans Ris, University of Wisconsin, Madison, WI

Grouping

This image is part of a group.

Imaging

Image Type
film
Imaging Mode
FBbi:00000622
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
visualization of contiguous regions
Processing History
unprocessed raw data

Sample Preparation

Methods
coagulating-fixative fixed tissue
critical_point dried specimen
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 2610px 1.5μm
Y 4750px 1.5μm
*CIL – Cell Image Library accession number. Please use this to reference an image.